Document Type
Conference Item
Publication Date
1-1-2007
Abstract
The microbial community that inhabit the thermophilic stage of composting process consist of complex thermophile communities that interact with each other to biodegrade lignocellulosic materials. The diversity of this community during oil palm empty fruit bunches (OPEFB) composting was investigated using PCR and denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. Compost samples were collected during the thermophilic phase with a temperature of between 60°C-62°C. Based on DGGE profile of 16S rDNA amplicons, four discrete bands were observed. Sequence analysis of the excised bands showed that sequences of these bands are closely related to those found in uncultured bacteria from other compost environments. The results suggest that the composting process of OPEFB during the thermophilic phase were dominated by yet-uncultured bacteria species.
Keywords
Thermophilic, Microbial species, rRNA gene, Ethidium bromide, Mutation
Divisions
InstituteofBiologicalSciences
Event Title
29th Symposium of the Malaysian Society for Microbiology
Event Location
Kuala Terengganu, Terengganu, Malaysia
Event Dates
24-26 November 2007
Event Type
conference
Additional Information
Conference paper