Document Type
Article
Publication Date
1-1-2010
Abstract
Quality control standardizations of the various medicinal plants used in traditional medicine is becoming more important today in view of the commercialization of formulations based on these plants. An attempt at standardization of Cassia spectabilis leaf has been carried out with respect to authenticity, assay and chemical constituent analysis. The authentication involved many parameters, including gross morphology, microscopy of the leaves and functional group analysis by Fourier Transform Infrared (FTIR) spectroscopy. The assay part of standardization involved determination of the minimum inhibitory concentration (MIC) of the extract which could help assess the chemical effects and establish curative values. The MIC of the C. spectabilis leaf extracts was investigated using the Broth Dilution Method. The extracts showed a MIC value of 6.25 mg/mL, independent of the extraction time. The chemical constituent aspect of standardization involves quantification of the main chemical components in C. spectabilis. The GCMS method used for quantification of 2,4-(1H,3H)-pyrimidinedione in the extract was rapid, accurate, precise, linear (R(2) = 0.8685), rugged and robust. Hence this method was suitable for quantification of this component in C. spectabilis. The standardization of C. spectabilis is needed to facilitate marketing of medicinal plants, with a view to promoting the export of valuable Malaysian Traditional Medicinal plants such as C. spectabilis.
Keywords
Cassia spectabilis standardization microscopy medicinal plants
Divisions
Dentistry
Publication Title
Molecules
Volume
15
Issue
5
Additional Information
ISI Document Delivery No.: 601YY Times Cited: 2 Cited Reference Count: 15 Cited References: Brain KR, 1975, PRACTICAL EVALUATION, P81 EVANS WC, 1996, TREASE EVANS PHARMAC, P545 GAEDCKE F, 2003, HERBAL MED PRODUCTS, P37 Lazarowych NJ, 1998, DRUG INF J, V32, P497 LORENZI H, 2002, PLANTAS MED BRASIL N, P291 National Committee for Clinical Laboratory Standards, 2002, M100S12 NCCLS Sangeetha S.ZurainiZ, 2008, INT J NAT ENG SCI, V2, P111 Sangetha S, 2008, Nihon Ishinkin Gakkai Zasshi, V49, P299, DOI 10.3314/jjmm.49.299 SANGETHA S, 2008, ASIAN J PHARM CLIN R, V1, P17 Sasidharan S, 2009, SAINS MALAYS, V38, P935 Soetardjo Surjani Jr, 2007, Malays J Nutr, V13, P81 Viegas C, 2004, J NAT PROD, V67, P908, DOI 10.1021/np0303963 World Health Organization, 1998, Quality controlmethods formedicinal plants Yadav NP, 2009, RECENT PROG MED PLAN, V24, P25 Yadav N. P., 2008, International Journal of Integrative Biology, V2, P195 Torey, Angeline Sasidharan, Sreenivasan Yeng, Chen Latha, Lachimanan Yoga University Sains Malaysia304/CIPPM/639040; Institute for Postgraduate Studies (IPS) of Universiti Sains Malaysia This project was funded by USM Short Term Grants (304/CIPPM/639040) from University Sains Malaysia. Angeline Torey is supported by the Universiti Sains Malaysia Fellowship Scheme from Institute for Postgraduate Studies (IPS) of Universiti Sains Malaysia. Molecular diversity preservation international-mdpi Basel