Document Type
Article
Publication Date
1-1-2010
Abstract
SAG2 is one of the major surface antigens of the intracellular protozoan parasite Toxoplasma gondii. In the present study, truncated recombinant SAG2(S) and full length recombinant SAG2(T) of T. gondii were optimally produced (similar to 15 mg/liter) in Pichia pus tons expression system using BMMY medium at pH 3, 25 degrees C in 0.5-1 methanol and a time-course of 1-2 days. The recombinant proteins were purified using a commercial gel filtration purification system obtaining similar to 33 recovery. The purified SAG2(S) and SAG2(T) showed molecular masses of 45 and 36 kDa by SDS-PAGE, respectively. The recombinant proteins were evaluated by Western blotting with patients' sera and demonstrated 90 sensitivity and 100 specificity for detection of toxoplasmosis. This study provided a means for large-scale expression and purification of SAG2, which should be useful for diagnosis of toxoplasmosis.
Keywords
Toxoplasma Gondii, Pichia Pastoris, Recombinant Antigen, Surface Antigen, Expression
Divisions
fac_med
Publication Title
The Southeast Asian Journal of Tropical Medicine and Public Health
Volume
41
Issue
3
Publisher
SEAMEO Regional Tropical Medicine and Public Health Network
Additional Information
Ling, Lau Yee Ithoi, Init Fong, Mun Yik