Document Type
Article
Publication Date
1-1-2010
Abstract
GeneFishing Discovery is a system applied on disease and gene related research for eukaryotes and the starting material for the study could be either total RNA or mRNA. In this study we demonstrate the use of this discovery system for a prokaryotic system by modifying the eukaryotic protocol using the poly (A)-tailing approach. As an example we compared the gene expression pattern of two closely related species of bacteria under the same family (Burkholderia thailandensis and Burkholderia cepacia) with satisfactory results.
Keywords
Genefishing, differential expression, prokaryotic system
Divisions
fac_med
Publication Title
African Journal of Biotechnology
Volume
9
Issue
35
Publisher
Academic Journals
Additional Information
Corresponding author. E-mail: khchua@um.edu.my. Tel: 603- 79676607. Fax: 603-79676600.
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