Identification of a Novel Second-Generation Circulating Recombinant Form (CRF48_01B) in Malaysia: A Descendant of the Previously Identified CRF33_01B
Document Type
Article
Publication Date
1-1-2010
Abstract
A molecular epidemiological investigation conducted among injecting drug users in eastern Peninsular Malaysia in 2007 identified a cluster of sequences (n = 3) located outside any known HIV-1 genotype. Analyses of near full-length nucleotide sequences of these strains from individuals with no recognizable linkage revealed that they have an identical subtype structure comprised of CRF01_AE and subtype B', distinct from any known circulating recombinant forms (CRFs). This novel CRF, designated CRF48_01B, is closely related to CRF33_01B, previously identified in Kuala Lumpur. Phylogenetic analysis of multiple CRF48_01B genome regions showed that CRF48_01B forms a monophyletic cluster within CRF33_01B, suggesting that this new recombinant is very likely a descendant of CRF33_01B. CRF48_01B thus represents one of the first examples of a "second-generation" CRF, generated by additional crossover with pre-existing CRFs. Corroborating these results, Bayesian molecular clock analyses indicated that CRF48_01B emerged in ~2001, approximately ~8 years after the emergence of CRF33_01B.
Keywords
Asia, Bayesian coalescent analysis, circulating recombinant form, CRF33_01B, CRF48_01B, injection drug user, Malaysia, phylogeny, time of the most recent common ancestor
Divisions
fac_med
Publication Title
Journal of Acquired Immune Deficiency Syndromes
Volume
54
Issue
2
Publisher
Lippincott, Williams & Wilkins
Additional Information
Li, Yue Tee, Kok Keng Liao, Huanan Hase, Saiki Uenishi, Rie Li, Xiao-Jie Tsuchiura, Takayo Yang, Rongge Govindasamy, Sumathi Yong, Yean Kong Tan, Hong Yien Pybus, Oliver G. Kamarulzaman, Adeeba Takebe, Yutaka