Brewery spent grain: chemical characteristics and utilization as an enzyme substrate

Document Type

Article

Publication Date

1-1-2010

Abstract

Grains in a brewery that have been removed of their sugar content is classified as a byproduct known as spent grain. It is a low-cost material and is available year-round. In Malaysia, approximately 15% of brewery spent grain (BSG) that is produced is salvaged and sold as feedstock supplement, while the remainder is discarded in landfills. This is a waste of a potentially useful resource. The chemical composition of fresh BSG was studied. Nine species of fungi were individually inoculated onto sterile BSG and solid state fermentation occurred without chemical adjustment or supplementation. Activity of lacease, lignin peroxidase, xylanase, amylase, carboxymethyl cellulase, protease and tannase was assayed. BSG consists of lignin and cellulose. High enzyme activity of lacease was shown by Pleurotus sajor-caju, Penicillium chrysogenum, and Aspergillus niger. This study is possibly the first to report lacease activity by solid state fermentation of spent grain.

Keywords

Brewery spent grain, Fungi, Hydrolytic enzymes, Solid state fermentation, Sustainable management, Waste reutilization

Publication Title

Malaysian Journal of Science

ISSN

1394-3065

Divisions

InstituteofBiologicalSciences

Volume

29

Issue

1

Publisher

Faculty of Science, University of Malaya

Additional Information

Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA

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