A simple, rapid and efficient method of isolating DNA from Chokanan mango (Mangifera indica L.)
Document Type
Article
Publication Date
1-1-2010
Abstract
Total DNA of Chokanan mango (Mangifera indica L.) was extracted from the leaf for the construction of total genomic library. However, the quality of the extracted DNA was often compromised by the presence of secondary metabolites, thus interfering with the analytical applications. Improvement on the quality of the extracted DNA was achieved through the optimization of leaf harvesting stage and modification on the cetyl trimethylammonium bromide (CTAB) DNA extraction procedure. Fully expended, soften and purplish leaf was proved to yield good DNA quality while the addition of polyvinylpyrrolidone (PVP) and beta-mercaptoethanol was effective in the removal of secondary metabolites, particularly polyphenolic compounds. The incorporation of high salt washing step was also efficient in removing polysaccharides. This simple, inexpensive and yet reliable method was proved to be successful in yielding sufficient quality and quantity of DNA for the construction of genomic library.
Keywords
Mango, DNA isolation, genomic library, secondary metabolites, polyphenolic compounds
Publication Title
African Journal of Biotechnology
Volume
9
Issue
36
Publisher
Academic Journals
Publisher Location
P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA