2-dodecanol (decyl methyl carbinol) inhibits hyphal formation and SIR2 expression in C. albicans
Document Type
Article
Publication Date
11-1-2009
Abstract
Candida albicans is capable of undergoing yeast-hypha transition to attain pathogenicity in humans. In this study, we investigated the differential expression of CaSIR2 via quantitative real-time PCR (qPCR), during yeast-hypha transition with and without the presence of 2-dodecanol. SIR2 transcript levels were found to be significantly enhanced after hyphal induction as compared to the yeast form. This study found that 2-dodecanol is able to inhibit hyphal development and block SIR2 up-regulation, even in hyphal-inducing growth conditions. We suggest that SIR2 may be involved in Candida albicans quorum-sensing and serum-induced yeast-hyphae transition via the Ras1-cAMP-Efg1 signalling cascade.
Keywords
Candida albicans, SIR2 gene, Quantitative real-time PCR, Gene expression, Dodecanol
Divisions
fac_med
Publication Title
Journal of Basic Microbiology
Volume
49
Issue
6
Publisher
John Wiley & Sons