The oxidative stress of hyperglycemia and the inflammatory process in endothelial cells.
Document Type
Article
Publication Date
2-1-2009
Abstract
Hyperglycemia and insulin resistance are common in many critically ill patients. Hyperglycemia increases the production of reactive oxygen species in cells, stimulates the production of the potent proinflammatory cytokines IL-8 and TNF-alpha, and enhances the expression of haem oxygenase-1, an inducible stress protein. It has been shown that administration of insulin and the semi-essential amino acid glutamine have been beneficial to the septic patient. The aim of our study is to test whether these two molecules, glutamine and insulin used in combination attenuate the proinflammatory responses in endothelial cells which have been triggered by hyperglycaemia. Our results demonstrate that a combination of insulin and glutamine are significantly more effective in reducing the expression of IL-8, TNF-alpha and HO-1 than insulin or glutamine alone.
Keywords
Cells, Cultured, Drug Therapy, Combination
Divisions
fac_med
Publication Title
The Journal of Medical Investigation
Volume
56
Issue
1,2
Publisher
The University of Tokushima, Faculty of Medicine
Additional Information
Department of Molecular Medicine, University of Malaya, Kuala Lumpur, Malaysia