Document Type
Article
Publication Date
1-1-2009
Abstract
Phototrophic hydrogen production by indigenous purple non-sulfur bacteria, Rhodopseudomonas palustris PBUM001 from palm oil mill effluent (POME) was optimized using response surface methodology (RSM). The process parameters studied include inoculum sizes ( v/v), POME concentration ( v/v), light intensity (klux), agitation (rpm) and pH. The experimental data on cumulative hydrogen production and COD reduction were fitted into a quadratic polynomial model using response surface regression analysis. The path to optimal process conditions was determined by analyzing response surface three-dimensional surface plot and contour plot. Statistical analysis on experimental data collected following Box-Behnken design showed that 100 (v/v) POME concentration, 10 (v/v) inoculum size, light intensity at 4.0 klux, agitation rate at 250 rpm and pH of 6 were the best conditions. The maximum predicted cumulative hydrogen production and COD reduction obtained under these conditions was 1.05 ml H2/ml POME and 31.71 respectively. Subsequent verification experiments at optimal process values gave the maximum yield of cumulative hydrogen at 0.66 ± 0.07 ml H2/ml POME and COD reduction at 30.54 ± 9.85. © 2009 International Association for Hydrogen Energy.
Keywords
Biohydrogen production, Photosynthetic bacteria, POME, Response surface methodology, Rhodopseudomonas palustris, Bacteria, Bacteriology, Gas producers, Light transmission, Optimization, Regression analysis, Sulfur, Surface analysis, Surface properties, Vegetable oils, Hydrogen production.
Divisions
fac_eng
Publication Title
International Journal of Hydrogen Energy
Volume
34
Issue
17
Additional Information
498TI Times Cited:18 Cited References Count:34