Document Type
Article
Publication Date
1-1-2009
Abstract
A recombinant His-Vi protein of Salmonella enterica serovar Typhi was successfully constructed and cloned into an expression vector through a nucleotide-nucleotide hybridization approach. After transformation of the construct into Escherichia coli, the recombinant His-Vi protein with a size of approximately 4 kDa was successfully produced and proven by Western blot analysis. This recombinant protein can be used to detect specific anti-Vi antibody produced by typhoid patients. Overall, the His-Vi recombinant protein could serve as a potential diagnostic reagent to detect S. Typhi infection in an individual.
Keywords
Salmonella enterica serovar Typhi, recombinant protein, Vi mimotope.
Divisions
fac_med,InstituteofBiologicalSciences
Publication Title
African Journal of Biotechnology
Volume
8
Issue
18
Publisher
Academic Journals
Additional Information
Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA