Document Type

Article

Publication Date

1-1-2009

Abstract

Purpose: To investigate the feasibility of using processed human amniotic membrane (HAM) to support the attachment and proliferation of chondrocytes in vitro which in turn can be utilised as a cell delivery vehicle in tissue engineering applications. Methods: Fresh HAM obtained from patients undergoing routine elective caesarean sections was harvested, processed and dried using either freeze drying (FD) or air drying (AD) methods prior to sterilisation by gamma irradiation. Isolated, processed and characterised rabbit autologous chondrocytes were seeded on processed HAM and cultured for up to three weeks. Cell attachment and proliferation were examined qualitatively using inverted brightfield microscopy. Results: Processed HAM appeared to allow cell attachment when implanted with chondrocytes. Although cells seeded on AD and FD HAM did not appear to attach as strongly as those seeded on glycerol preserved intact human amniotic membrane, these cells to be proliferated in cell culture conditions. Conclusion: Preliminary results show that processed HAM promotes chondrocyte attachment and proliferation.

Keywords

Amnion, Chondrocytes, Tissue Engineering, Cell Carrier

Publication Title

Malaysian Orthopaedic Journal

ISSN

1985-2533

Divisions

fac_med

Volume

3

Issue

2

Publisher

University of Malaya

Additional Information

Department of Orthopaedic Surgery, University of Malaya, Kuala Lumpur, Malaysia. ------------------> Corresponding Author: Boo Lily, Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia Email: boolily83@gmail.com

Download

Share

COinS