Spectroscopic studies of chlorophyll a from Opuntia ficus-indica
Document Type
Article
Publication Date
1-1-2009
Abstract
Green pigment obtained from Opuntia ficus-indica intact plant was solvent extracted with absolute ethanol. The pigment solution was subjected to various photoexcitations at wavelengths of 222, 308, 337 and 448 nm. Fluorescence from the excited state exhibit peaked in the regions 450-485 nm and 650-680 nm. Upon comparison with the literature, the green solution is chlorophyll a. Fluorescence intensity ratio ( blue to red) differs with excitation wavelengths. It is known that chlorophyll a fluoresces at 673 nm. From the absorption spectrum, the peaks at similar to 404, similar to 501, similar to 605 and similar to 663 nm correspond to the singlet 4 (S4), singlet 3 (S3), singlet 2 (S2), and singlet 1 (S1) peaks, respectively.
Keywords
Chlorophyll Opuntia ficus-indica Fluorescence Absorption spectroscopy
Divisions
InstituteofBiologicalSciences
Publication Title
Materials Research Innovations
Volume
13
Issue
3
Publisher
Springer Verlag (Germany)