Document Type
Article
Publication Date
1-1-2006
Abstract
Ammonium uptake kinetics of low specific growth rate cultures of Pseudomonas putida PGA1, a gram negative fluorescent pseudomonad, which is an important producer of poly(3-hydroxyalkanoates) (PHA) molecules, were studied. The ammonium uptake follows a first-order kinetics model, indicating that the micro-organism’s specific uptake rate of ammonium and its growth would increase as the ammonium ion concentrations become higher. Optimal ammonium uptake and growth rates were observed at 0.1 gL-1 ammonium ion in aqueous medium. It also indicated that the ammonium transport mechanism is probably inducible by ammonium concentration. The limit of ammonium ion level increase before cells stop growing completely is given by the substrate inhibition constant, Ki which equals to 1.2 gL-1 ammonium ion concentration.
Publication Title
Asia Pacific Journal of Molecular Biology and Biotechnology
ISSN
0128-7451
Recommended Citation
Annuar, Mohamad Suffian Mohamad; Tan, Irene Kit Ping; Ibrahim, Shaliza; and Ramachandran, K.B., "Ammonium uptake and growth kinetics of pseudomonas putida PGA1" (2006). Research Publications (2006 to 2010). 187.
https://knova.um.edu.my/research_publications_2006_2010/187
Divisions
InstituteofBiologicalSciences
Volume
14
Issue
1
Publisher
Malaysian Society for Molecular Biology and Biotechnology