Document Type
Article (Restricted)
Publication Date
1-1-2008
Abstract
A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1 mRNA expression in histamine-treated (10-7M to 10-9 M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohisto-chemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations.
Keywords
Bovine retinal endothelial cells, Zonula Occludens-1, Real time reverse transcription poly-merase chain reaction
Divisions
fac_med
Publication Title
Biomedical Research
Volume
19
Issue
2
Publisher
Biomedical Research Press
Additional Information
Department of Medicine, Faculty of Medicine, University of Malaya