Document Type
Article (Restricted)
Publication Date
1-1-2008
Abstract
A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1 mRNA expression in histamine-treated (10-7M to 10-9 M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohisto-chemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations.
Keywords
Bovine retinal endothelial cells, Zonula Occludens-1, Real time reverse transcription poly-merase chain reaction
Publication Title
Biomedical Research
ISSN
0388-6107
Recommended Citation
Ismail, I.S.; Muniandy, S.; Koon, C.Y.; Bee, J.C.; Rayappan, A.V.; Kum, T.W.; and Qvist, R., "Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR" (2008). Research Publications (2006 to 2010). 1611.
https://knova.um.edu.my/research_publications_2006_2010/1611
Divisions
fac_med
Volume
19
Issue
2
Publisher
Biomedical Research Press
Additional Information
Department of Medicine, Faculty of Medicine, University of Malaya