Document Type

Article

Publication Date

1-1-2008

Abstract

A kinetic model is presented giving a mathematical description of batch culture of Pseudomonas putida PGA1 grown using saponified palm kernel oil as carbon source and ammonium as the limiting nutrient. The growth of the micro-organism is well-described using Tessier-type model which takes into account the inhibitory effect of ammonium at high concentrations. The ammonium consumption rate by the cells is related in proportion to the rate of growth. The intracellular production of medium-chain-length poly-(3-hydroxyalkanoates) (PHAMCL) by P. putida PGA1 cells is reasonably modeled by the modified Luedeking-Piret kinetics, which incorporate a function of product synthesis inhibition (or reduction) by ammonium above a threshold level.

Keywords

Ammonium, Kinetic, Medium-chain-length PHA, P. Putida, Substrate-inhibition, Batch cell culture, Biochemical engineering, Biochemistry, Cell culture, Chain length, Kinetic theory, Batch cultures, Carbon sources, Chain lengths, Consumption rates, High concentrations, Hydroxyalkanoates (PHA), Inhibitory effects, Intracellular production, Kinetic modelling, Mathematical descriptions, Palm kernel oil (PKO), Product synthesis, Pseudomonas putida, Threshold levels, Ammonium compounds

Divisions

fac_eng

Publication Title

Brazilian Journal of Chemical Engineering

Volume

25

Issue

2

Publisher

Brazilian Society of Chemical Engineering

Additional Information

307HK Times Cited:6 Cited References Count:19

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