The effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus I.) merr cv. smooth cayenne) shoot-tip culture
Document Type
Article
Publication Date
1-1-2008
Abstract
Seven different hormone treatments, namely 6-benzylammopunne (BAP) at 2, 3 mg L-1 was applied singly and in combination with Indole Acetic Acid (IAA) at 0.18, 0.8 and 1.8 mg L-1, BAP at 3.3 mg L-1 in combination with IAA at 1.8 and 3.3 mg L-1 and triple combination of BAP at 2.3, IAA at 1.8 and Gibberellic acid (GA3) at 1.0 mg L -1 were tested, over four different incubation periods of 30, 45, 60 and 75 days, for their effect in the proliferation and growth of Smooth cayenne pineapple shoot-tip culture. Combined application of BAP at 3.3 and IAA at 1.8 mg L-1 induced the highest proliferation of 19 shoots/explant and the highest total of 121 and 125 shoots over 4 cycles of multiplication. Raising the IAA to 3.3 mg L-1 resulted in the lowest proliferation and stunted shoots. Incorporation of GA3 improved the shoot length but caused drastic reduction in proliferation. The other treatments showed an intermediate effect. © 2008 Asian Network for Scientific Information.
Keywords
Ananas comosus In vitro proliferation Incubation period length Pineapple Shoot production phytohormone article growth, development and aging plant Ananas Plant Growth Regulators Plant Shoots
Divisions
Science
Publication Title
Pakistan Journal of Biological Sciences
Volume
11
Issue
3