Studies on plant regeneration and somaclonal variation in Saintpaulia ionantha Wendl. (African Violet)
Document Type
Article
Publication Date
1-1-2008
Abstract
Efficient plant regeneration of Saintpaulia ionantha (African violet) has been obtained in the present study. MS medium supplemented with 1.0 mg L -1 IAA and 2.0 mg L-1 Zeatin resulted in 100 shoot regeneration and induced the highest number of shoots (average 15.0±0.8 shoots per explant) after being cultured for 8 weeks. The above hormone combination was optimum for shoot regeneration. Most of Saintpaulia ionantha plantlets derived from tissue culture system could be hardened and transferred to the greenhouse conditions with 84.0±1.6 success rate. However, regenerated plantlets of Saintpaulia ionantha (even after 12 months old) failed to flower. Morphological characters of regenerated plantlets of Saintpaulia ionantha were observed and compared with in vivo (intact) plants. Regenerated plantlets showed some differences in morphological characters, such as height and leaf size, texture and colour, but the plantlets showed no variation in leaf arrangement and leaf margin. However, the morphological characters of the regenerated plantlets were found to be unstable. © 2008 Asian Network for Scientific Information.
Keywords
Morphogenesis Organogenesis Plantlets Saintpaulia ionantha Somaclonal variation Vegetative propagation indoleacetic acid derivative phytohormone angiosperm article genetics histology metabolism physiology regeneration Angiosperms Indoleacetic Acids Plant Growth Regulators
Divisions
InstituteofBiologicalSciences
Publication Title
Pakistan Journal of Biological Sciences
Volume
11
Issue
9
Additional Information
Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA