Document Type
Article
Publication Date
1-1-2004
Abstract
A gene encoding the larval excretory-secretory antigen TES-120 of the dog ascarid worm Toxocara canis was cloned into the methylotrophic yeast Pichia pastoris. Specificity of the recombinant TES-120 antigen produced by the yeast was investigated. Forty-five human serum samples from patients infected with differentparasitic organisms, including 8 cases of toxocariasis, were tested against the recombinant antigen in immunoblot assays. Results from the assays showed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.
Keywords
Human Toxocariasis
Divisions
fac_med
Publication Title
Parasitology Research
Volume
92
Issue
2
Publisher
Springer Verlag (Germany)
Additional Information
M.-Y. Fong (&) Æ Y.-L. Lau Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: fongmy@um.edu.my Fax: +60-3-79674754