Document Type
Article
Publication Date
1-1-2004
Abstract
A gene encoding the larval excretory-secretory antigen TES-120 of the dog ascarid worm Toxocara canis was cloned into the methylotrophic yeast Pichia pastoris. Specificity of the recombinant TES-120 antigen produced by the yeast was investigated. Forty-five human serum samples from patients infected with differentparasitic organisms, including 8 cases of toxocariasis, were tested against the recombinant antigen in immunoblot assays. Results from the assays showed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.
Keywords
Human Toxocariasis
Publication Title
Parasitology Research
Recommended Citation
Fong, M.Y. and Lau, Y.L., "Recombinant expression of the larval excretory-secretory antigen TES-120 of Toxocara canis in the methylotrophic yeast Pichia pastoris" (2004). Research Publications (2000 to 2005). 951.
https://knova.um.edu.my/research_publications_2000_2005/951
Divisions
fac_med
Volume
92
Issue
2
Publisher
Springer Verlag (Germany)
Additional Information
M.-Y. Fong (&) Æ Y.-L. Lau Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: fongmy@um.edu.my Fax: +60-3-79674754