Document Type
Article
Publication Date
1-1-2003
Abstract
We report a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Salmonella isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns by independent researchers showed that subtyping results using this modified, rapid protocol were highly reproducible.
Divisions
InstituteofBiologicalSciences
Publication Title
Malaysian Journal of Science
Volume
22
Issue
2
Publisher
Faculty of Science, University of Malaya
Additional Information
Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
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