Document Type
Article
Publication Date
1-1-2003
Abstract
The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES- 120 antigen produced by the bacterium was investigated.A total of 45 human serum samples from patients infected with different helminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.
Keywords
Parasitology
Divisions
fac_med
Publication Title
The Southeast Asian Journal of Tropical Medicine and Public Health
Volume
34
Issue
4
Publisher
SEAMEO Regional Tropical Medicine and Public Health Network
Additional Information
Correspondence: Dr Mun-Yik Fong, Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Fax: 603-79674754 E-mail: fongmy@um.edu.my