Document Type
Article
Publication Date
1-1-2003
Abstract
The gene encoding the excretory-secretory antigen TES-120 of dog ascarid worm Toxocara canis was cloned into the bacterium Escherichia coli. The specificity of the recombinant TES- 120 antigen produced by the bacterium was investigated.A total of 45 human serum samples from patients infected with different helminthes and protozoa, including 8 cases of toxocariasis, were tested against the recombinant antigens in immunoblot assays. The results from the assays revealed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.
Keywords
Parasitology
Publication Title
The Southeast Asian Journal of Tropical Medicine and Public Health
ISSN
0125-1562
Recommended Citation
Fong, Mun Yik; Lau, Y.L.; Ithoi, Init; Jamaiah, I.; Anuar, A.K.; and Rahmah, N., "Recombinant expression of Toxocara canis excretory-secretory antigen TES-120 in Escherichia coli" (2003). Research Publications (2000 to 2005). 707.
https://knova.um.edu.my/research_publications_2000_2005/707
Divisions
fac_med
Volume
34
Issue
4
Publisher
SEAMEO Regional Tropical Medicine and Public Health Network
Additional Information
Correspondence: Dr Mun-Yik Fong, Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Fax: 603-79674754 E-mail: fongmy@um.edu.my