Document Type
Article (Restricted)
Publication Date
1-1-2002
Abstract
Dengue virus type 2 (DENV-2) infection induced apoptotic cellular DNA fragmentation in Vero cells within 8 days of infection. The addition of high concentrations of extracellular Zn2+ but not Ca2+, Mg2+ or Mn2+ to the cell culture medium hastened the detection of apoptosis to within 4 h after infection. No apoptotic cellular DNA fragmentation was detected in the cell culture treated with Zn2+ alone or infected with heat- or ultraviolet light-inactivated DENV-2 in the presence of Zn2+. These results suggest that (i) apoptosis is induced in African green monkey kidney cells infected with live DENV-2 and (ii) the addition of high extracellular Zn2+ accelerates detection of apoptosis in the DENV-2-infected cells. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Keywords
Animals Apoptosis/*drug effects/*physiology Cercopithecus aethiops Dengue Virus/*physiology In Situ Nick-End Labeling Vero Cells Zinc/*pharmacology
Divisions
fac_med
Publication Title
Febs Letters
Volume
524
Issue
1-3
Additional Information
Shafee, Norazizah AbuBakar, Sazaly eng Research Support, Non-U.S. Gov't Netherlands 2002/07/24 10:00 FEBS Lett. 2002 Jul 31;524(1-3):20-4.