Cellular behavior in embryogenic and non-embryogenic sugar beet calluses

Document Type

Article

Publication Date

1-1-2005

Abstract

The present study discusses the results of cytological studies of two kinds of sugar beet callus, i.e., embryogenic and non-embryogenic tissues. The calluses were produced through culture of secondary leaves on Murashige and Skoog medium containing two hormone combinations. One week after transfer of calluses onto fresh medium, their cells were viewed using electron microscopy and an image analyzer. Observations showed that cells of the two callus types had considerable differences in cell structure and various organelles. Of note were the high amount of polyploidization, rough endoplasmic reticulum, polysome, poly-nucleolus, and incomplete cell wall together with abnormal partitioning in non-embryogenic cells, as compared to embryogenic cells. In contrast, vacuolation of cytoplasm, perfect cell wall and partitioning structure, and the high proportion of nucleus/cytoplasm area were recognized in embryogenic cells.

Keywords

Beta vulgaris L. electron microscopy image analysis somatic embryo ultrastructure beta-vulgaris-l neoplastic progression somatic embryogenesis plant-regeneration cells growth calli l.

Publication Title

In Vitro Cellular and Developmental Biology - Plant

ISSN

1054-5476

Divisions

Science

Volume

41

Issue

4

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