A DNA detection system based upon a high TcSQUID and Ultra-small magnetic particles
Document Type
Article
Publication Date
1-1-2005
Abstract
A high Tc SQUID system for biological molecules (DNA) detection is developed. This system is based on a hybridization process. Two strands in a DNA molecule are held together by hydrogen bonds between base pairs like a ladder. The two strands are referred to as being complementary each other. HPVB 33 (Human Papillomavirus Probes 33) was prepared as a DNA. One strand (Sample DNA) was labeled with Fe3O4 ultra-small magnetic particles and the other (probe DNA) was anchored on a glass slide. Then they were hybridized each other on the slide. After washing the excess sample DNA, the hybridized DNA was evaluated in the presence of excitation ac field by high Tc SQUID. The signal was initially proportional to the concentration of the sample DNA and then saturated. It means that the hybridization occurred successfully between the sample DNA and the probe DNA.
Keywords
hybridization, magnetic detection, medical applications, small particles, squid, molecules
Divisions
PHYSICS
Publication Title
IEEE Transactions on Applied Superconductivity
Volume
15
Issue
2
Publisher
Institute of Electrical and Electronics Engineers (IEEE)
Additional Information
Department of Physics, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA