Date of Award

1-1-2000

Thesis Type

Masters

Document Type

Thesis

Divisions

Institute for Advanced Studies

Department

-

Institution

Universiti Malaya

Abstract

In this study, the yield of biomass and polyhydroxyalkanoate  (PHA) of Pseudomonas oleovorans grown in batch and fed-batch cultivations were compared. When oleic acid (QA) (0.5% w/v) was used as carbon source, a significant increase in biomass.and PHA content were observed through fedbatch cultivation. The biomass and PHA content obtained from batch cultivation were 1.45±0.19 g/I and 2.50±0.77 % cell dry weight (CDW) respectively, while in the fed-batch cultivation, the biomass and PHA content increased significantly to 3.35±0.78 g/I and 32.72±0.30 %CDW respectively.  When saponified palm olein (SPO) (0.5% w/v) was used as a carbon source, the batch cultivation yielded 1.54±0.03 g/I biomass and 3.40±0.76 %CDW of PHA. In the fed-batch cultivation, there was no significant increase in biomass yield (1 .9±0.66 g/I) but there was a significant increase in PHA content (14.46±1.18 %CDW). In the batch cultivation, yield of PHA (Y p1,), from OA and SPO were 8.05 mg/g and 10.47 mg/g respectively. The polymer obtained when either OA or SPO was used as a carbon source was a medium chain length PHA (MCLPHA) with hydroxyoctanoic acid (Ca) being the major monomer. Palm oil-mill effluent (POME) was chosen as the source to isolate and screen bacteria capable of utilising palm oil directly for growth and PHA accumulation. Bacterial isolates were screened by using Sudan Black B and Nile Blue A staining. Out of 45 isolates, none was capable of utilising palm oil directly for growth. Isolate X4.13 was however, able to grow and accumulate PHA from SPO. When isolation was carried out by an enrichment technique, one isolate, FLP1, was found to be able to grow and accumulate PHA directly from crude palm oil, palm olein, palm stearin, palm kernel oil and oleic acid. The superior isolate was identified as Pseudomonas sp. The PHA was then analysed to be polyhydroxybutyrate (PHB), a short chain length PHA (SCLPHA).  Thus, palm oil appears to be a suitable carbon source which could be converted to MCLPHA as well as SCLPHA depending on the bacteria metabolising the oil.

Initial

snms

Additional Information

Dissertation (M.A) -- Institute for Advanced Studies, Universiti Malaya, 2000

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