Date of Award
2010
Thesis Type
Masters
Document Type
Dissertation
Divisions
Faculty of Science
Department
Institute of Biological Sciences
Institution
Universiti Malaya
Abstract
MicroRNAs (miRNAs) are small single-stranded non-protein-coding RNAs of about 22 nucleotides that play an important role in post-transcriptional gene regulation in animals and plants by targeting mRNAs for direct cleavage of mRNAs or repression of mRNA translation. In this study, a homology search based approach has been used for identifying the novel miRNA genes of Musa sp. using the BLAST program with a total of 42,978 Musa sp. expressed sequence tags (EST) were compared to a total of 959 previously known plant miRNA sequences. Forty-seven candidate miRNA with homology to previously known miRNAs were identified. By using the mFold3.1 program, secondary RNA structures were predicted and total of nine novel miRNA were identified in Musa sp. One of the most promising novel miRNA was miR156 which has the strongest characteristics of a miRNA precursor structure and was 100% identical to the mature miR156 from other plant species. It was experimentally validated to be expressed in Musa acuminata var. Berangan and is the first known report of the identification and expression of miRNA from a banana plant species.
Additional Information
Dissertation (M.A.) – Faculty of Science, Universiti Malaya, 2010.
Recommended Citation
Yew Seong, Tan, "Computational identification of micro RNA in MUSA sp EST sequences." (2010). Student Works (2010-2019). 232.
https://knova.um.edu.my/student_works_2010s/232
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
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