Date of Award

2010

Thesis Type

Masters

Document Type

Dissertation

Divisions

Faculty of Science

Institution

Universiti Malaya

Abstract

Two candidate yield-related genes, putative Receptor-like Protein Kinase 1 (putative RPK1) and putative CLAVATA1 Receptor Kinase (putative CLV1), were selected for molecular characterization and expression analysis. This was carried out using in four rice lines comprising the parents and two BC2F7 lines from a local rice breeding program, with Malaysian rice variety, Oryza sativa ssp. indica cv. MR219 as recurrent parent, and Oryza rufipogon (IRGC105491) as donor parent. Approximately 2.8 kb and 2.6 kb putative RPK1 cDNA nucleotide sequences were obtained from Oryza rufipogon and Oryza sativa ssp. indica cv. MR219 respectively. However, only 402 bp partial cDNA nucleotide sequence of putative CLV1 were isolated from Oryza rufipogon and Oryza sativa ssp. indica cv. MR219. Both of the genes were identified and categorised into the transmembrane receptor kinase group, which represents the largest group of plant RLK/Pelle family. Real time quantitative reverse transcriptase Polymerase Chain Reaction (qRT-PCR) analysis indicated that putative CLV1 was highly up-regulated during booting stage and heading stage; whereas, putative RPK1 was up-regulated at booting stage. Based on Southern hybridization analysis, one to three copies of putative RPK1 and three to five copies of putative CLV1 are present in the genome of Oryza rufipogon and Oryza sativa ssp. indica cv. MR219. About one hundred base pair of putative RPK1 gene sequence and putative CLV1 gene sequence respectively were used to assemble plant RNA interference constructs. The RNAi vector containing sequences of putative CLV1 was successfully introduced into Oryza rufipogon, IRGC105491 by Agrobacterium-mediated transformation.

Initial

khm

Additional Information

Dissertation (M.A.) – Faculty of Science, Universiti Malaya, 2010.

Download

Share

COinS