Date of Award
2010
Thesis Type
Masters
Document Type
Dissertation
Divisions
Faculty of Science
Department
Department of Physics
Institution
Universiti Malaya
Abstract
Bacteriorhodopsin is a photoactive protein found in the purple membranes of the highly halophilic bacterium Halobacterium Salinarium. Due to highly efficient and simple in its function, Bacteriorhodopsin has been extensively researched and successfully incorporated into many practical applications. In this regard, the fabrication of compactly packed and functionally active Bacteriorhodopsin thin film is crucial in realizing a highly efficient device. The aim of this thesis is to analyze the mechanical and thermodynamic properties of the Bacteriorhodopsin-Langmuir monolayer film from the surface pressure-area per molecule isotherm to produce a compactly pact and functionally active monolayer film. For this, we have proposed and utilized the forced mixing technique to mix Bacteriohodopsin solution and hexane to produce a complex spreading emulsion without the use of sonication. Different concentration of Bacteriorhodopsin solutions were prepared and forced mixed with hexane to study the effect of Bacteriorhodopsin solution concentration on the hysteresis between the annealing and full isotherm. It was discovered that 1mg/ml concentration of Bacteriorhodopsin solution showed minimal or no hysteresis. Therefore the 1mg/ml concentration was used for the analysis of mechanical and thermodynamic properties of the Bacteriorhodopsin monolayer film. From the analysis, we found that Bacteriorhodopsin form a thermodynamically and mechanically optimal packing density at surface pressure of 22 mN/m. Based on this surface pressure, Bacteriorhodopsin monolayer film was deposited onto an Indium Tin Oxide slide and subjected to various characterization methods. The light-adapted absorption peak at 568nm indicates successful generation of photocycle process in the reconstituted monolayer films. The Auger Electron Scanning and Surface Profiler showed that the deposited film is 4.5 nm thick. In this work we showed that the suggested deposition surface pressure from the thermodynamic and mechanical analysis produces a compactly packed and functionally active Bacteriorhodopsin monolayer film that can be utilized for future biosensor works.
Additional Information
Dissertation (M.A.) – Faculty of Science, Universiti Malaya, 2010.
Recommended Citation
Kuppusamy, Vijayandren, "Mechanical and thermodynamic properties of bacteriorhodopsin langmuir film." (2010). Student Works (2010-2019). 141.
https://knova.um.edu.my/student_works_2010s/141
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